ࡱ > > @ ; < = [ 8 bjbj ΐ ΐ W W W k k k 8 | , k ; r K K K K K ) ) ) : : : : : : : $ ? A D ; 9 W * ) ^ ) * * ; K K P O; / / / * 8 K K : / * : / / : ,4 , ! 6 4 K / k 0- X4 : e; 0 ; b4 . A - A 4 4 d A W 5 ) " * / 7* K* ) ) ) ; ; d. ) ) ) ; * * * * A ) ) ) ) ) ) ) ) ) : M o n o c l o n a l a n t i - h u m a n U B E 2 L 6 a n t i b o d y ( c l o n e k 1 H 3 ) M o u s e I g G 2 b , k C a t . N o . A U B 0 9 0 7 S i z e : 1 0 0 l C a t . N o . A U B 0 9 5 7 S i z e : 5 0 l I m m u n o g e n : R e c o m b i n a n t h u m a n U B E 2 L 6 ( 1 - 1 5 2 a a ) p u r i f i e d f r o m E . c o l i N C B I A c c e s s i o n N o . : N P _ 0 0 4 2 1 4 I s o t y p e : M o u s e I g G 2 b h e a v y c h a i n a n d k l i g h t c h a i n C l o n e : A n t i - h u m a n U B E 2 L 6 m A b , c l o n e k 1 H 3 , i s d e r i v e d f r o m h y b r i d i z a t i o n o f m o u s e F 0 m y e l o m a c e l l s w i t h s p l e e n c e l l s f r o m B A L B / c m i c e i m m u n i zed with a recombinant human UBE2L6 protein. Description: Ubiquitin-conjugating enzyme E2L6 (UBE2L6), also known as UbcH8, is a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination of a protein substrate requires the concerted action of 3 classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin protein ligases. The E2 ubiquitin-conjugating enzyme is highly similar in primary structure to the enzyme encoded by UBE2L3 gene. Concentration: 1 mg/ml Form: Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide Storage: Can be stored at 4C for up to one month, but store at -20C for long term storage. Avoid repeated freezing and thawing cycles. Usage: The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 3,000. Recommended starting dilution is 1:2,000. Application: ELISA, WB General references : Ardley HC., et al. (2000) Cytogenet Cell Genet. 89(1-2):137-140 Movnihan TP., et al. (1999) J Biol Chem. 274(43):30963-30968 Kumar S., et al. (1997) J Biol Chem. 272(21):13548-13554 Western blot analysis Cell lysates of MCF7 (2 " $ . : < \ d f h j t v ŰkRJ;$ ,hi h B*CJ OJ QJ ^J aJ o(ph h CJ OJ QJ ^J aJ o(h t h o(1h h B*CJ" KH OJ PJ QJ ^J aJ" ph )h t 5B* CJ" OJ QJ ^J aJ" o(ph 4h h B*CJ" KH OJ PJ QJ ^J aJ" o(ph )h1 5B* CJ" OJ QJ ^J aJ" o(ph )hXP 5B* CJ" OJ QJ ^J aJ" o(ph )h, 5B* CJ" OJ QJ ^J aJ" o(ph ,h h 5B* CJ" OJ QJ ^J aJ" ph h 5CJ( OJ QJ ^J o( h j B g e W \0UDVD]\^0 R kd $$If c |$ ( t (6 4 4 l ap d $G$ If WD ` $If $$4$7$ 8$ H$ If a$gd dx $G$ If UDzVDzWD;]^` v | ~ ˹wsbSD3333 hD h CJ OJ QJ ^J aJ hU CJ OJ QJ ^J aJ o(h~*} CJ OJ QJ ^J aJ o( hD hD CJ OJ QJ ^J aJ h h CJ OJ QJ ^J aJ h CJ OJ QJ ^J aJ o('hD h CJ OJ PJ QJ ^J aJ o( hD h CJ OJ QJ ^J aJ #hD h CJ OJ QJ ^J aJ o( hMa CJ H*OJ QJ ^J aJ o( hmP CJ H*OJ QJ ^J aJ o( #hx h[ CJ OJ QJ ^J aJ o( " $ 6 8 < @ B D H X ` b d j p_pP?P?P h 5CJ OJ QJ ^J aJ o( h 5CJ OJ QJ ^J aJ h> 5CJ OJ QJ ^J aJ o( hU 5CJ OJ QJ ^J aJ o( h~*} 5CJ OJ QJ ^J aJ o( #hD hD 5CJ OJ QJ ^J aJ #hD h 5CJ OJ QJ ^J aJ hD h 5o( hD h o('hD h CJ OJ PJ QJ ^J aJ o( hD h CJ OJ QJ ^J aJ #hD h CJ OJ QJ ^J aJ o( j z 2 B D h ʿ}pbU h9 5OJ QJ ^J o( hv h OJ QJ ^J o(h 6OJ QJ ^J o( h~*} OJ QJ ^J o(hmP OJ QJ ^J o(hl9 OJ QJ ^J o(h1 OJ QJ ^J o(h OJ QJ ^J o(h[ OJ QJ ^J o(h OJ QJ ^J o(h 5OJ QJ ^J o( h o(h h 5CJ OJ QJ ^J aJ h 5CJ OJ QJ ^J aJ o(B D . / z { h i \0UDVD]\^0gdQ \0UDVD]\^0gdp1b \0UDVD]\^0gd \0UDVD]\^0 h l ~ ƻxk]SH= h1 OJ QJ ^J o(h[ OJ QJ ^J o(h OJ QJ ^J h 5OJ QJ \^J o(h 5OJ QJ \^J hmP h OJ QJ ^J o(hD h OJ QJ ^J hMa H*OJ QJ ^J o( hmP H*OJ QJ ^J o( hD h OJ QJ ^J o(h OJ QJ ^J o(h 5OJ QJ ^J o( hx hua. OJ QJ ^J o(h~*} h~*} OJ PJ QJ ^J hua. hua. 5OJ QJ ^J o( 0 2 : # $ - . / ; < > Y f ɾԦzm[L; h^; OJ QJ \^J mH o(sH hW OJ QJ \^J mH sH #hG hG OJ QJ \^J mH sH h/! 5OJ QJ ^J o( h/! h/! 5OJ PJ QJ ^J "hW h 5OJ PJ QJ ^J o( hl9 OJ QJ ^J h1 OJ QJ ^J o(h 5OJ QJ \^J h OJ QJ ^J o(hj3 OJ QJ ^J o(h t OJ QJ ^J o(h[ OJ QJ ^J o(h OJ QJ ^J h OJ QJ ^J o( f u v w { | G % ν{naTE4 h+ hB2S OJ QJ ^J mH sH hB2S OJ QJ ^J mH o(sH hB2S hB2S OJ QJ ^J hB2S hp1b OJ QJ ^J hp1b hp1b OJ QJ ^J h;@ h;@ OJ QJ ^J mH sH h<+ OJ QJ ^J mH o(sH h+ h<+ OJ QJ ^J mH sH hW hG OJ QJ ^J mH sH h^; OJ QJ \^J mH o(sH #hW hW OJ QJ \^J mH sH hW OJ QJ ^J mH o(sH hG hG OJ QJ ^J mH sH % S n s y z { İpaPF;F;F; h. OJ QJ ^J o(h. OJ QJ ^J h;F h OJ QJ ^J mHsH h KH OJ PJ QJ ^J o(hov OJ QJ ^J mHo(sHh OJ QJ ^J mHsH h mHo(sHh 5mHo(sH h 5OJ QJ ^J mHsH'h+ hAt B*OJ QJ \^J o(ph hB2S hp1b 5OJ QJ ^J h^; OJ QJ ^J mH o(sH hB2S OJ QJ ^J mH sH hB2S hB2S OJ QJ ^J mH sH " $ _ f h i p ! " } λΡ}r}h h9 OJ QJ ^J h OJ QJ ^J o(h9 OJ QJ ^J o(hP OJ QJ ^J o(hX hP OJ QJ ^J o(hX h OJ QJ ^J h 5OJ QJ \^J h OJ PJ QJ ^J o( h+%1 ^J h OJ QJ ^J h 5OJ QJ \^J o(h;F h. OJ QJ ^J o(h OJ QJ ^J o( ' 阊|u^QD hE 5OJ QJ ^J o( h 5OJ QJ ^J o( ,j hgF 5OJ QJ U^J mH nH o(u h PJ o(j h UmH nH u j hpP| UmH nH u hg h OJ QJ ^J o(h h 5OJ QJ ^J h+%1 OJ QJ ^J o(h+%1 OJ QJ ^J ht OJ QJ ^J hX OJ QJ ^J o(h OJ QJ ^J h OJ QJ ^J o(h t OJ QJ ^J o( ; x gdE WDv`gdv $ /VDWD^ `/a$gdv $ 04$7$ 8$ H$ VD^0a$ gdE $ 4$7$ 8$ H$ a$ gdE |VD>]^| \0UDVD]\^0gdg ) + - . 1 2 3 6 7 8 : ; F I ̽̽o̽b̽b̽WF hFc h[y OJ QJ ^J mHsH h[y OJ QJ ^J o(h:t3 CJ aJ mHo(sHhW}A 5CJ \aJ mHo(sHhW}A 6CJ ]aJ mHo(sHh:t3 CJ aJ mHsH h:t3 6CJ ]aJ mHo(sH"hq= h:t3 6CJ ]aJ mHsH hq= h:t3 CJ aJ mHsH hW}A CJ aJ mHo(sHhW}A CJ aJ o(hm( hu- 5CJ \aJ hm( hu- 5CJ \aJ o( I O Q U W b d g h j l q r w x Ͼ옾ϾϾϊnZL=n hFc OJ QJ ^J mHo(sHhFc OJ QJ ^J mHsH &hFc hFc 6OJ QJ ]^J mHsH hFc hFc OJ QJ ^J mHsH hFc OJ QJ ^J o(h[y h[y OJ QJ ^J o(#h[y 5OJ QJ \^J mHo(sH&hFc h[y 6OJ QJ ^J mHo(sH hFc h[y OJ QJ ^J mHsH h[y OJ QJ ^J mHo(sHh[y OJ QJ ^J mHsH &hFc h[y 6OJ QJ ]^J mHsH Ƶ~~~kV (h 5B*OJ PJ QJ \^J o(ph %h 5B*OJ PJ QJ \^J ph h h o(h(u j h(u U hiz@ o(hFc hiz@ OJ QJ ^J o(hFc OJ QJ ^J mHo(sH hFc hFc OJ QJ ^J mHsH #hFc 5OJ QJ \^J mHo(sH&hFc hFc 6OJ QJ ]^J mHsH &hFc hFc 6OJ QJ ^J mHo(sH 7$ 8$ H$ 8 8 8 8 8 /8 18 P8 U8 V8 W8 \8 ȲqqaqK5 *h1 B*CJ OJ PJ QJ ^J aJ o(ph *h$] B*CJ OJ PJ QJ ^J aJ o(ph h B*OJ PJ QJ ^J ph *h B*CJ OJ PJ QJ ^J aJ o(ph 'h B*CJ OJ PJ QJ ^J aJ ph -hj h B*CJ OJ PJ QJ ^J aJ ph *hK B*CJ OJ PJ QJ ^J aJ o(ph U"h B*OJ PJ QJ ^J o(ph "hK B*OJ PJ QJ ^J o(ph "h6Z B*OJ PJ QJ ^J o(ph 0ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human UBE2L6 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. \8 ]8 `8 a8 b8 8 8 8 8 8 8 ʴ hiz@ o(h *h B*CJ OJ PJ QJ ^J aJ o(ph *h B*CJ OJ PJ QJ ^J aJ o(ph *h6Z B*CJ OJ PJ QJ ^J aJ o(ph 'h B*CJ OJ PJ QJ ^J aJ ph h OJ QJ ^J o( 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 gdE H$ 4 1h2P /R . A!"#$% S@= 'k|Bm4 l 9 8\ xyT\ۖmR^xAB=&)4 !x