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Rabbit IgG SmartBlot is immunoblotting detection reagents, which react
strongly with native rabbit IgG
primary antibodies used for detecting specific antigens on Western blots without
binding to the reduced and
denatured rabbit IgG heavy chain band (50 kD) and light chain band (25 kD),
which come from the rabbit
primary antibody used for immunoprecipitating the antigens of interest from cell
lysates or protein mixtures.
Therefore, by using our Rabbit IgG SmartBlot, detection of antigens with
molecular weights near 50 kD or 25
kD is not obscured by large amounts of reduced and denatured rabbit IgG heavy
chains and light chain on the
blots.
Highlights:
EFFECTIVE – Dramatically reduces the noise
of heavy chain and light chain observed when
Western Blotting immunoprecipitated proteins.
SIMPLE – No changes to your existing
immunoprecipitation and western blot protocols.
COMPATIBLE –Use of anti-rabbit IgG beads
for immunoprecipitation.
Products:
Rabbit IgG SmartBlot-HRP: va-4000-001, 50ul
Rabbit IgG SmartBlot-AP: va-4000-002, 50ul
Rabbit IgG SmartBlot-Biotin: va-4000-003, 50ul
Store at 4° C and stable for six months from the
date of shipment.
Related Products:
Goat IgG SmartBlot-HRP: va-6000-001
Goat IgG SmartBlot-AP: va-6000-002
Goat IgG SmartBlot-Biotin: va-6000-003
Western Blot Signal Enhancer: va-100
Blocking Reagent: va-99
Luminol Reagent: va-98
Research Use:
For research use only, not for use in diagnostic
procedures.
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