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Semicarbazide Elisa

Semicarbazide (SEM) ELISA Test Kit

Price :€ 576                 art.code: 202-SEM1-E

1. Principle

This test kit from Shenzhen Lvshiyuan Biotechnology Co.;Ltd is based on the competitive enzyme immunoassay for the detection of semicarbazide (SEM) in the tissue(chicken, fish, shrimp), milk, honey. The conjugate antigen is pre-coated on the micro-well stripes. The SEM in the testing sample competes with the conjugate antigen pre-coated on the micro-well stripes, to interact with the antibody against SEM. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of SEM in the sample. This value is compared to the standard curve and the content of the corresponding SEM is subsequently obtained.

2. Technical specifications

Sensitivity : 0.1ppb

Detection limit:

Tissue and honey———————-0.2ppb

Recovery rate

Tissue (shrimp and fish) ————85±10%

Honey ,chicken meat /liver——— 75±15%

Cross-reaction rate:

SEM——————100%

AHD  ————— ﹤0.1%

AMOZ ————— ﹤0.1%

AOZ  ————— ﹤0.1%

96 wells l/kit

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Last modified: 05/29/09