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Europe
GENTAUR
 +32
1658 9045
 +32
1650 9045
info@gentaur.com
Av. de l' Armée 68
B-1040 Brussels
BELGIUM
France
tel 01 43 25 01 50
fax01 43 25 01 60
9, rue Lagrange
75005 Paris
Italy
tel 02 36 00 65 93
fax 02 36 00 65 94
20135 Milano
Germany
tel +32
1658 9045
fax +32
1650 9045
Japan
tel +81 78 386 0860
fax +81 78 306 0296
Minaatojimaminami-manchi
Chuo-ku, Kobe
065-0047
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- CometAssay™ Control
Cells
Trevigen’s CometAssay™ Control Cells are a set of cell
preparations containing
different levels of DNA damage to be used with Trevigen’s CometAssay™ Kits. In a
typical comet assay, electrophoresis methods and differences in cell
preparations create
a significant source of variation in comet tail parameters. Such variation
sometimes
makes it difficult to compare results between laboratories, and even within the
same
lab. To overcome this problem, Trevigen scientists developed a set of stable
control
cell populations containing incremental levels of DNA damage for use when
performing
the CometAssay™. These control cells, when electrophoresed in the CometAssay™,
consistently produce comets with predetermined percents of DNA in the tails. The
healthy control cell population (CC0) was treated with Etoposide under various
conditions to increase the amount of damage in populations CC1, CC2, and CC3,
respectively. These cryopreserved control cells are designed to act as standards
to
compare alkaline electrophoresis methods between individual users and
laboratories.
Damage Measurement for Control Cells
 
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Control Cells
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n
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Mean
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SD
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CCO
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302
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3.748
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9.6864
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CC1
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300
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23.737
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14.6832
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CC2
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211
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39.190
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19.4772
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CC3
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235
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57.122
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24.4672
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Features |
Benefits |
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First available set of qualified control
cells for use with the comet assay |
Standardize and validate alkaline
electrophoresis methods between individual users and laboratories
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Includes four populations: one population
of healthy cells and three populations with increasing amounts of DNA
damage |
Provides a range of DNA damage for
comparison, which meets the needs of most laboratories. Damaged
populations show statistically distinct differences |
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Cells are cryopreserved
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After initial thaw, aliquot and refreeze,
the cells remain stable in liquid nitrogen for long term storage
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Qualified for alkaline gel
electrophoresis |
Compatible with the electrophoresis
method used by most laboratories |
- Ordering Information:
CometAssay™ Controls
- 4256-010-CC
ControlAssay™ Control Cells
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- Related Products:
CometAssay™ Kits
- 4250-050-K
CometAssay™ Kit 4251-050-K
- CometAssay™
Silver Kit 4254-200-K
CometAssay™
- Silver Staining Kit 4252-040-K
CometAssay™HT
- Sample Kit 4253-096-K
CometAssay™ Kit 96 Wells
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CometAssay™ Slides
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4250-050-03 |
CometSlideTM
(2-well slide) |
25 slides |
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4250-200-03 |
CometSlideTM
(2-well slide) |
100 slides |
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4250-500-01 |
CometAssayTM
HT Slide (20 well slide) |
25 slides |
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4250-02K-01 |
CometAssayTM
HT Slide (20 well slide) |
100 slides |
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4253-960-03 |
96-Well CometSlideTM
(96 well slide) |
10 slides |
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4253-02K-03 |
96-Well CometSlideTM
(96 well slide) |
20 slides |
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4253-10K-03 |
96-Well CometSlideTM
(96 well slide) |
100 slides |
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