GENTAUR
+32 1658 9045
or
0032 (0)16 41 44 07
+32 1650 9045
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Other Countries
0032 (0)16 41 44 07
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K200 K180
K181 K160
K161
K200 Total IgE
EIA
Total immunoglobulin E (IgE) serum level is widely reported as the laboratory
marker of atopic diseases
such as atopic asthma, atopic dermatitis, and pollenosis. Separately, high
levels of total serum IgE
are characteristic for parasitic infestations and some other clinical disorders
including superficial and
systemic mycosis. Decreased levels of IgE are found in cases of
hypogammaglobulinemia, autoimmune
diseases, ulcerative colitis, and primary biliary cirrhosis.
In allergic patients, serum total IgE level in general corresponds to the
severity of the allergic disease
and may be therefore used for monitoring of all kinds of anti-allergic therapy
or allergen elimination.
Sample type: serum, plasma
Incubation: 30’/30’/15’, 370С
Control sample: 1
Sample volume: 50 μl
Calibrators: 5 (0-1000 IU/ml)
Shelf life: 12 months
Sensitivity: 6 IU/ml
Normal range, IU/ml: <130
K180 Gliadin
IgG EIA
K181 Gliadin IgA EIA
Celiac disease (CD) or gluten-sensitive entheropathy is a chronic disease
characterized by impared
intestinal absorption due to mucosal lesions. The exact ethiology of CD is
unknown but it is clearly
shown that gliadin - the alcohol soluble fraction of wheat gluten - is the toxic
agent. High concentrations
of antigliadin antibodies (AGA) found in blood, saliva and intestinal secretions
are characteristic for untreated
celiac patients. These antibodies gradually disappear after gluten exclusion
from the patient’s diet.
AGA testing is a simple and inexpensive method to efficiently select candidates
for mucosal biopsy of the
duodenal-jejunal junction, the latter method being essential for the
confirmation of the diagnosis of CD.
Early detection of AGA in high risk populations would contribute to prevent the
insidious consequences
of chronic malabsorption. Individuals at risk include short-stature children,
unexplained anemia,
unexplained hypocalciemia or osteomalacia, delayed puberty, insulin-dependent
diabetes mellitus,
autoimmune thyroiditis and selective IgA deficiency. It is well established that
IgA-AGA are more
specific than IgG-AGA. Nevertheless, combined IgG/IgA screening might be more
effective since there
is an unexplained but clear association between CD and selective IgA deficiency.
In treated celiac patients without this deficiency IgA-AGA is the test of choice
for monitoring diet
compliance. Serum IgA-AGA level responds very quickly to the admission of
gluten-free diet (levels
drop below the cut-off level within two to six months) while IgG-AGA may take
more than one year to
become negative; breaking the diet causes more prompt elevation of IgA-AGA
compared to IgG-AGA.
Herpetiform dermatitis is a disease entity strongly associated to
gluten-sensitive enteropathy, and AGA
serology is not capable to distinguish between these two diseases.
Separately, there are well reported clinical conditions showing positive IgG-AGA
and, rarely, IgA-AGA
non-related to histologically proven CD, eg all kind of malapsortion syndromes,
including Crohn’s
disease, ulcerative colitis, galactosidase deficiency, post-infection
malapsorption etc. The patients
with rheumatoid arthritis, Sjogren syndrome, systemic sclerosis and other
connective tissue diseases
show abnormally high prevalence of moderately elevated gliadin IgA and IgG.
These findings may be
considered as non-relevant to GI pathology; however, gliadin free diet may be
implemented for their
treatment.
According to data obtained by Xema, first degree relatives of CD children
patients (especially their
parents) show very frequent positivity for both IgG-AGA and IgA-AGA while
displaying virtually no
symptoms of gastrointestinal diseases, herpetiform dermatitis, or other diseases
supposed to cause
this positivity.
Test flowchart, sensitivity, calibrators and normal ranges are the same in both
kits (please, see below).
Sample type: serum, plasma
Sensitivity: 10 U/ml
Control sample: 1
Sample predilution: 1:101
Incubation: 30’/30’/15’, RT
Shelf life: 12 months
Sample volume: 100 μl
Calibrators: 5 (0-200 U/ml)
Normal range, U/ml: <25
K160 Anti-tTG IgG
EIA
New kit!
K161 Anti-tTG IgA EIA
New kit!
Celiac disease (CD) is a serious, lifelong, gastrointestinal disorder that can
cause a wide spectrum
of clinical symptoms in children and adults. CD patients are unable to digest
gluten, present in all
crops, especially in wheat, barley and rye.The classic symptoms are diarrhea,
abdominal distension,
weight loss and malnutrition. Those symptoms were often misdiagnosed and
interpreted to be due
to gastroenteritis, food allergy, viral or other infection, anemia, stress,
nervous condition, irritable
bowel and food allergy. For this reason the proper diagnosis of CD could take
several years.
The various serological tests employed in the work-up of patients suspected to
have CD include antigliadin
antibody (AGA), anti-endomysial antibody (EMA), anti-reticulin antibody (ARA)
and anti-tissue
transglutaminase (tTG) antibody tests. Antibodies to gliadin and tTG are
detected by ELISA, whereas
endomysium and reticulin antibodies are detected by indirect immunofluorescence.
EMA are very specific indicators of CD. However, the EMA test is an
immunohistochemical method that
requires experience in reading immunofluorescence reactions.
Since identification of tTG as the endomysial antigen, ELISA methods have been
described for detecting
antibodies in the sera of patients with CD. The advantage of the anti-tTG
antibody assay is that it is less
subjective than EMA and more accurate than AGA assay. In various studies on the
efficacy of the antitTG
antibody method for screening for CD, the specificity and sensitivity of this
method has been found
to range from 90 percent to 95 percent. Anti-tTG antibodies also quickly
disappear after admission
of gluten-free diet (within 6 months). Test flowchart, sensitivity and
calibrators are the same in both
kits (please, see below).
Sample type: serum,
Sensitivity: 5 U/ml
Control sample: 1
Sample predilution: plasma 1:101
Incubation: 30’/30’/15’, 370С
Shelf life: 12 months
Sample volume: 100 μl
Calibrators: 6 (0-200 U/ml)
Normal range, U/ml: <20 (IgA), <30 (IgG)
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