- SAINT-MIX for the transfection of Embryonic Stem cells

 
Recent SAINT-MIX Articles:

Geiger T, Plosone 2008 Feb Vol 3(2):e1574 Anomalous Features of EMT during
Keratinocyte Transformation.(Transfection was performed with SAINT-MIX
(Synvolux Therapeutics B.V.) according to the manufacturer instructions)

Shafiei F, Oncogene 2008 Vol 27, 2602-2612. DNMT3A and DNMT3B mediate autocrine
hGH repression of plakoglobin gene transcription and consequent phenotypic conversion
of mammary carcinoma cells.(Saint-Mix (Synvolux Therapeutics B.V., The Netherlands)
transfection reagent was used according to the manufacturer’s instructions…)

Gommans WM, Mol Carcinog. 2007 May;46(5):391-401 Engineering zinc finger protein
transcription factors to downregulate the epithelial glycoprotein-2 promoter as
a novel anti-cancer treatment. (Transfection of plasmids using SAINT
(Synvolux Therapeutics, Groningen, The Netherlands)).

 
SAINT-MIX Applications:

DNA transfection:
A wide variety of mammalian cells can be transfected with SAINT-MIX.
SAINT-MIX has been used to transfect several cell-types/lines, some with efficiencies up to 100%.

Oligonucleotides:
SAINT-MIX has been demonstrated to act as an efficient translocator of oligo-nucleotides into eukaryotic cells. The compatibility with serum is a major advantage of this delivery system. Oligonucleotide induced cell-damage will be minimal.

 
SAINT-MIX product description and protocols.

The SAINT-MIX delivery system is based on a cationic pyridinium head group, showing excellent bio-compatibility. Molecular structure of SAINT-18 (1-methyl-4-(cis-9-dioleyl) methyl-pyridinium-chlorid):
 

SAINT-MIX consists of SAINT and DOPE (Dioleoyl-phosphatidylethanolamine) dissolved in H2O. The SAINT-18: DOPE ratio is 1:1.

Stability experiments showed that SAINT-MIX, stored at 4°-7°C, is stable for at least one year after opening. No loss of function appeared after repeatedly opening of the vials as long as the vials are recapped tightly after use. The antibiotic properties of SAINT-MIX ensure sterility for prolonged periods.