8-Wellx12 strips (antibody- coated)

8 wells

12 strips

Anti-HMGB1 polyclonal antibody

Standard (lyophilized)                                    

1 mL

1

Pig HMGB1

Positive control (lyophilized)                             

1 mL

1

Pig HMGB1

Sample diluent solution

20 mL

1

Buffer containing additives and preservative

Peroxidase-linked antibody(lyophilized)

10 mL

1

Peroxidase-linked anti-HMGB1,2 monoclonal antibody

Peroxidase-linked antibody dissolvent solution

10 mL

1

Buffer containing additives and preservative

Substrate solution A

5 mL

1

3,3', 5,5'-Tetramethyl-benzidine, dihydrochloride, dihydrate

Substrate solution B

5 mL

1

Buffer containing 0.005 mol/L hydrogen peroxide

Stop solution

10 mL

1

0.35 mol/L sulfuric acid

Wash solution (5x)

100 mL

2

5-Fold concentrated buffer containing Tween 20

Plate seal                                  

-

2

Data Sheet