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Cultrex® BME marked
“PathClear” do not have LDEV intact virus or fragments of the virus present as
tested for by supersensitive PCR. No virus present means that the researcher’s
animals cannot become infected. Rather than passively screening and selecting
lots in which the virus is not detected, each lot of PathClear™ BME is
manufactured by methods developed by to actively remove LDEV and other
pathogens. Having control over the virus removal process assures customers of a
continuous supply of pathogen free BME.
In addition to LDEV, PathClear™ BME is tested and cleared of 30 other pathogens
and viruses by PCR.
Certificate of Analysis
Cat # |
Product Name |
Quantity |
Selling Price |
3430-001-02 |
Cultrex® BME
with phenol red PathClear™ |
1 ml |
95,5 € |
3430-005-02 |
Cultrex® BME
with phenol red PathClear™ |
5 ml |
247,6 € |
3431-001-02 |
Cultrex® BME
Reduced Growth Factor with phenol red PathClear™ |
1 ml |
95,5 € |
3431-005-02 |
Cultrex® BME
Reduced Growth Factor with phenol red PathClear™ |
5 ml |
281,4 € |
3432-001-02 |
Cultrex® BME
without phenol red PathClear™ |
1 ml |
95,5 € |
3432-005-02 |
Cultrex® BME
without phenol red PathClear™ |
5 ml |
247,6 € |
3433-001-02 |
Cultrex® BME
Reduced Growth Factor without phenol red PathClear™ |
1 ml |
95,5 € |
3433-005-02 |
Cultrex® BME
Reduced Growth Factor without phenol red PathClear™ |
5 ml |
281,4 € |
3444-005-02 |
Cultrex® High
Protein Concentration BME PathClear™ |
5 ml |
304,8 € |
3460-024-K |
CultreCoat® BME Coated Cell Invasion Assay |
2 plates |
563,5 € |
Cultrex®
endothelial cell invasion kit accelerates the screening process for compounds
that influence vascular endothelial cell digestion through and migration across
the extracellular basement membrane. This kit offers a flexible, standardized,
high-throughput format for quantitating the degree to which endothelial cells
penetrate a barrier of basement membrane extract (BME), in vitro, in response to
chemoattractants and/or inhibiting compounds. This assay utilizes a Boyden
chamber with a BME coated 8 micron polyethylene terephthalate (PET) membrane.
Sulfuraphane, a naturally occurring chemopreventive agent, is provided as a
control for inhibition of in vitro endothelial cell migration/invasion
on/through the basement membrane. Detection of cell invasion is quantified using
Calcien AM.
Laminin is an extracellular matrix protein which contains a number of functional
domains that allow it to assemble into sheets. Trevigen's mouse laminin is
purified from EHS sarcoma.
The PathClear®
designation means that in addition to standard sterility, endotoxin and MAP
testing, the Laminin is tested by PCR and is clear of 31 pathogens and viruses,
including LDEV. Each lot is rigorously qualified in biological performance
assays.
Features: |
Pathogen Free Laminin - Tested and cleared of 31
pathogens and viruses including LDEV as well as bacteria and mycoplasma. |
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Applications: |
·
Cell adhesion
·
Cell outgrowth
·
Cell to cell interaction |
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Preparation: |
Mouse laminin is provided sterile in DMEM medium with 10
µg/ml gentamycin sulfate, at a concentration of 1 mg/ml. |
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Performance Testing: |
Trevigen's mouse laminin is tested for activity in a
neurite outgrowth assay. |
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Source: |
Purified from murine EHS sarcoma. |
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Storage: |
Store at -80°C in a manual defrost freezer. Avoid
repeated freeze-thaw cycles. |
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