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TrueVision is a unique and highly effective reagent that dramatically
improves the sensitivity and
signal-to-noise ratio of immunohistochemical staining, allowing you to clearly
visualize cellular targets
that would normally indistinguishable due to low level target expressions or
high background
staining.
Highlights:
Enhance your staining detection
signal
Boost your immunohistochemical staining
signal up to 10-fold.
Promote antibody-antigen interaction
Work through a novel mechanism by
increasing antigen accessibility in
immunohistochemical staining
Easy to use
Ready to use reagent and no need to change
your current protocol. Simply dilute your
antibodies with TrueVision reagent.
Product:
Each bottle contains 20ml of the reagent. Store at
4° C and stable for three months from the date of
shipment.
Protocol:
1. Sample preparation
a. Tissue culture cells are grown on glass slides and fixed
with acetone or others.
b. Frozen tissue blocks are sliced 4 to 10 micron thick. The
sections are adhered to glass slides and
fixed with acetone or others
(Optional: Incubate for 5–10 minutes
in 0.1–1% hydrogen peroxide in PBS to quench endogenous
peroxidase activity)
2. Immunohistochemical staining
a. Block the samples with your blocking reagents
b. Incubate the samples with primary antibodies diluted in
TrueVision reagent for 60 min,
wash with TBS
c. Incubate the samples with fluorescence conjugated
secondary antibodies diluted in
TrueVision reagent for 60min. Wash
with TBS
Or
Incubate the samples with HRP
conjugated secondary antibodies diluted in TrueVision
reagent for 60 min, wash with TBS
d. If HRP conjugated secondary antibodies are used, apply DAB
for 5 to 30min staining. Wash the
samples with TBS and counterstain.
e. Mount
Related products:
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kit
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detection kit
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Chemokine products
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